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Cote : N-622 Auteur f s) : F. MEYER Date : Novembre 1979 Titre : Quantitative analysis of the chromatin of lymphocytes : an essay on comparative structuralism Résumé : With 26 letters we can form ail the words we use, and with a few words it is possible to form an infinité number of différent meaningful sentences. In our case, the letters will be a simple neighborhood image transformations and area measurements. The paper shows how, by iterating these transformations, it is possible to obtain a good quantitative description of the nuclear structure of Feulgen-stained lymphocytes (CLL and normal). The fact that we restrict ourself to a small number of image transformations made it possible to construct an image analysis System able to do these transformations very quickly (TAS). We will see, successively, how to segment the nucleus itself, the chromatin and the interchromatinic channels, how openings and closings lead to size and spatial distribution curves, and how skeletons may be used for measuring the lengths of interchromatinic channels. Sommaire : I. Introduction 3 II. Material 5 III. Methods 5 1/ Segmentation of the whole nucleus 5 2/ Segmentation of the chromatin 9 3/ The segmentation of the interchromatinic channels 11 4/ Densitometric measurements 12 5/ Granulometric measurements 12 6/ Spatial distribution of the chromatin particles 15 7/ Width and length of the interchromatinic channels 16